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Poster: Rapid and Scalable Transient Transfection Technology for High Titer Protein Production in HEK, CHO and Other Cell Types.
Applications > Protein Production > Insect Cell Transfection
The MaxCyte STX uses a proprietary, scalable electroporation technology to rapidly (co)transfect a variety of cell types, including insect cells, with DNA, RNA, siRNA, proteins or other biomolecules of interest. The MaxCyte STX can perform small-scale transfections of 5x105 cells in seconds for use in basic research and assay development or perform bulk transfections of up to 1x1010 cells in less than 30 minutes for use in protein production.
MaxCyte Electroporation for Insect Cell Transfection:
- High level transfection efficiency & cell viability
- Simple, reproducible transfection
- Fully scalable, able to transfect up to 1x1010 cells in < 30 minutes
High Efficiency Transfection of Insect Cells
SF9 and SL3 insect cells have been transfected using MaxCyte scalable electroporation. Figure 1 shows that SL3 transfection efficiencies are greater than 90% and that the level of GFP expression (mean fluorescence intensity) is dependent on the DNA concentration used during transfection. No DNA toxicity was evident at the highest DNA concentration as cell viability levels were the same for cells transfected with 0, 100, or 200µg/mL DNA. Figure 2 describes the transfection of the SF9 insect cell line. 24 hours post electroporation, 55% of cells expressed GFP when analyzed via FACS.
Figure 1. Protein Expression in SL3 Insect Cells. SL3 cells were transfected with varying concentrations of pIEX4GFP. Cell viability and GFP expression were assessed 1 day post EP using microscopy and FACS analysis. Transfection efficiencies exceeded 90% for cells transfected with either 100 or 200 μg/mL DNA. Mean fluorescence intensity (MFI) correlated with DNA concentration, illustrating how MaxCyte STX users can control transgene expression levels by varying loading agent concentrations.
Figure 2. Protein Expression in SF9 Insect Cells. SF9 cells were transfected with 200μg/mL of pIEX4GFP. GFP expression was assessed 24 hours post electroporation via FACS analysis.