Reporter Gene Assay

Simple to Develop, Simple to Scale Reporter Gene Assays

 

Rapidly develop and scale up reporter gene assays for a wide range of druggable targets including multi-subunit and/or toxic proteins. Run your reporter assays within hours of transfection or cryopreserve cells for future use.

  • Proven performance in reporter assays including NF-kB , NFAT, and dual reporter systems
  • Rapid assay development and scale up
  • Bulk cell transfections with ability to cryopreserve cells
  • Cell type flexibility to create more biologically relevant cell systems
  • Does not require special expression constructs, engineered cells or specialized reagents

 

 

Straightforward Assay Development & Scale Up

Cytokine Receptor Stimulation Elicits Dose-dependent Luciferase Expression. A. HEK 293 cells were transfected with 3 different concentrations of the pGL4.32 (NF-kB) plasmid. 4 hours post electroporation, cells were treated with TNFa and incubated overnight.  Luciferase activity was measured using Promega Bright-Glo®. B. Cells were transfected via small- or large-scale electroporation and cryopreserved.  Cells were thawed and treated with TNFa 4 hours after plating.  Luciferase activity was measured following an overnight incubation.  Error bars denote standard deviations from 6 replicate wells. 

Create Assay Ready Cells Through Cryopreservation

NFAT reporter gene assay sensitivity is dependent on the [DNA] used for transfection.  A. HEK 293T cells were transfected with 3 concentrations (0.5, 1, and 2mg/1E6 cells) of the pGL4.30 (NFAT reporter) plasmid.  24 hours after plating, cells were treated (+/-) with Induction Solution (10 ng/ml PMA, 1uM ionomycin) and incubated overnight.  Luciferase activity was measured using Promega Bright-GLO®.   B. HEK cells were transfected with 1.5mg/1E6 cells pGL4.30 and the cells cryopreserved.  Transfected cells were thawed, and treated (+/-) with Induction Solution 4 hours after plating.  Luciferase activity was measured following an overnight incubation.  Error bars denote standard deviation from 3 replicate wells.

 

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