siRNA

Highly Efficient siRNA Delivery to Relevant Cell Types

 

Rapidly delivery siRNA or siRNA/protein complexes to your cells of interest with high efficiency and minimal cell disturbance for superior results.

  • Able to (co)transfect a variety of molecules including mRNA, siRNA, DNA & proteins
  • High transfection efficiency and cell viability
  • Efficient delivery to primary cells, stem cells, & other historically difficult-to-transfect cell types
  • Does not require chemicals or specialized reagents

 

 

Greater Than 98% Loading of Hematopoietic Stem Cells

 

Isolated human CD34+ cells were transiently transfected with FITC-labeled siRNA.   Control cells were incubated with siRNA, but not electroporated.  Transfected and control (incubated) cells were examined via FACS. Greater than 98% of electroporated cells were positive for both FITC and CD34, while less than 1% of non-electroporated cells were positive for FITC.

Gene Silencing in MCF-7 Breast Cancer Cells

 

MCF-7 cells were transiently transfected with siRNA targeting the estrogen receptor (ESR1), GATA-3 or control siRNA (targets GFP).  A. Images were taken 2 days post MCF-7 cell transfection under phase contrast microscope showing morphological cell changes. B. Immunofluorescent staining of MCF-7 cells either mock transfected or transfected with ESR1 siRNA.  Cells were fixed and stained two days after transfection.  Left panels, DAPI staining; right panels stained with anti-ESR1 antibody.  C. MCF-7 cells were collected on 1 and 3 days post electroporation and total proteins isolated for western blot analysis.  Data courtesy of National Human Genome Research Institute.

Surface CD45 Knockdown in Primary Macrophages

Isolated macrophages were transfected with siRNA targeting the CD45 surface receptor using either the MaxCyte STX or via lipid-mediated delivery.  Two concentrations (0.1 and 10uM) of the siRNA were transfected via MaxCyte electroporation.  Cells were examined 20, 30 and 66 hours post transfection via FACS for CD45 surface expression.

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