Non-therapeutic Gene Editing

Flexibility Meets Performance for Efficient Gene Editing

Looking to rapidly perform gene editing and eliminate viral vectors? Deliver RNAs and/or RNA/protein components of your preferred nuclease system to most all cell types, including historically difficult-to-transfect cells like primary cells, with the push of a button. Perform gene insertion, deletion or correction, genome modification, and stem cell generation & differentiation in your laboratory using a non-viral delivery platform that meets the stringent demands of clinical use, but has the flexibility to meet all your research needs.

  • Rapid engineering of cell lines, human primary and stem cells, and induced pluripotent stem cells (iPSCs)
  • High cell viability with minimal cell disturbance
  • High efficiency (co)transfection of DNA, RNA and proteins
  • Proven compatibility with CRISPR, ZFN, and TALEN nuclease systems using a non-viral delivery method
  • Easy-to-use, computer-controlled platform

 

Explore the Maxcyte Difference

 

Efficient, Consistent Delivery of CRISPR Components

 

K562 cells were electroporated with mRNA-CRISPR (Cas9 and guide RNAs). Products of a corrected AAVS-1 site are 298 and 170 base pairs, the parental band is 468 base pairs. The editing rate was calculated as (digested bands)/(digested bands + parental band).

Increasing Protein Production Through CHO Genome Modification

 

 

 

CRISPR-mediated Integration of Protein Expression Construct within CHO Genome. CHO-S cells were transfected with two ratios of donor plasmid to Cas9 & gRNA. Selection was applied 72 hours post electroporation. Cells electroporated with either ratio of CRISPR components recovery within 11 days. Fifteen of 30 clones isolated from stable pools showed locus-specific integration by PCR.

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