Cell Lines

Cell Type Efficiency% GFP +
(24-48 hr)
Viability
(% PI -)
MRC-5 75% 95%
B16F10 80% 90%
VERO 90% 90%
Renca 80% 97%
CHO Cells 90% 90%
NIH 3T3 90% 90%
Mouse Embryonic Fibroblasts 95% 95%
Jurkat 90% 90%
K562 90% 90%
Huh-7 Cells 80% 90%
A20 60% 80%
293M 85% 85%
293T 95% 95%

Results of processing cell lines with DNA plasmid encoding for Green Fluorescent Protein (GFP).
Efficiency expressed as % cells GFP+ at 24 to 48 hrs post process; viability as % cells excluding
propidium iodide (PI).