MaxCyte VLX®

The MaxCyte VLX® Large Scale Transfection System is a small-footprint, easy-to-use instrument specifically designed for extremely large volume transient transfection. Using proprietary flow electroporation technology, the MaxCyte VLX can transfect up to 2E11 cells in less than 30 minutes with high cell viability and transfection efficiencies. This cGMP-compliant system is ideal for the rapid production of recombinant proteins, monoclonal antibodies, viral vectors, vaccines & VLPs from the bench through cGMP pilots and commercial manufacturing.

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What if you could produce grams of protein without constructing a single stable cell line?

The MaxCyte VLX Large Scale Transfection System can transiently transfect up to 2E11 cells in a single 30 minute transfection run. Optimized electroporation protocols designed specifically to maximize protein expression enable multi-gram level production from even historically difficult-to-transfect cells such as CHO cells. The MaxCyte VLX uniquely meets the speed, reproducibility and safety needed for manufacturing of human therapeutics and makes protein production via transient transfection a viable alternative to the development of stable cell lines.

 
Plasmid
eGFP
EPO-IRES-eGFP
Volume of Cells
380 mL
1 L
Number of Cells
3.8 x 1010
2.2 x 1010
Cell Viability
95%
97%
eGFP+
82%
-
EPO Production
(IU/106 cells)
-
17
High-quality Transfection using the MaxCyte VLX. Cells grown in a 25L WAVE Bioreactor™ were harvested, washed and resuspended in MaxCyte electroporation buffer. Cells were transfected via flow electroporation using the MaxCyte VLX at the designated volumes with either an eGFP or EPO-IRES-eGFP expression vector. Cell viability, GFP expression and erythropoietin (EPO) production were assessed.
MaxCyte VLX Specs

High efficiency transfection using pre-loaded cell-type specific electroporation protocols

MaxCyte transfection efficiencies are routinely greater than 90% and cell viability greater than 90% (Figure 2). MaxCyte technology causes minimal off-target perturbations of gene expression, resulting in assays with higher fidelity and improved efficiency. MaxCyte instruments enable transfection of a variety of cell lines, historically difficult-to-transfect cells, primary cells, and stem cells using preprogrammed electroporation protocols. Standard electroporation protocols provide an optimal blend of loading efficiency and cell viability. MaxCyte scientists have developed additional protocols for CHO and HEK cells that are designed specifically for high level protein expression. There are currently over 30 different electroporation protocols and our list is continually expanding. MaxCyte scientists are available to consult with clients to identify the most appropriate protocol for any cell type.

High Efficiency, High Viability Transfection.
Figure 2. High Efficiency, High Viability Transfection. Ten different cell types were transfected using MaxCyte electroporation with 200μg/ml pGFP DNA using the appropriate pre-loaded protocol. 24 hrs post transfection cells were examined for cell viability (% cells excluding propidium iodide) and transfection efficiency (% GFP+ cells).
Current MaxCyte Electroporation Protocols
A549 CV-1 L5278Y NS0 SH-SY5Y
B16 DLD-1 LNCaP Panc-1 SK-MES-1
BHK-21 H1299 MCF7 PC12 SK-N-SH
C2C12 HEK 293 MDA-MB-231 PC-3 SL3
C6 Hela MDCK Primary Fibroblasts SW403
CaCo-2 Hep G2 Mesenchymal Stem Cells RBL THP-1
CAP/CAP-T HOS Min-6 Renca U2OS
CHO Huh-7 Neuro2a RLE U937
COS-1 Jurkat NIH 3T3 SF21 Vero
Cos-7 K562 NIH3T3L1 SF9