Flow Electroporation

Any Cell. Any Molecule. Any Scale.®

MaxCyte’s Flow Electroporation™ Technology is an enabling technology with superior performance, flexibility and scalability which allow it to uniquely fulfill the demands of drug discovery, protein production and cell therapy applications.

 

What makes Flow Electroporation Technology Unique?

  • Unmatched scalability – half a million (0.5 x 106) up to 200 billion (2 x 1011) cells
  • Research and clinical-grade technology
  • Broad cell type compatibility including primary and stem cells
  • Superior transfection performance – high transfection efficiency and cell viability
  • Does not require specialized constructs, engineered cells, reagents or media additives
  • Uses a single, chemically-defined electroporation buffer for all cells

 

 

Flow Electroporation Basics

 

Flow Electroporation™ Technology is based on a fundamental principle of cell membranes ­– the reversible permeability of membranes in the presence of an electrical field – thereby creating a universal transfection technology capable of high-performance delivery of virtually any molecule(s), including DNA, RNA, proteins and cell lysates, to any cell type with minimal cell disturbance.

Flexibility Meets High-Performance

 

Our scientists have developed robust electroporation protocols optimized for more than 80 different cell types as well as protocols that can be used to identify the optimum parameters for virtually all other cell types. Flow Electroporation Technology transfects any cell type using a single, chemically-defined electroporation buffer. It causes minimal cell disturbance, routinely resulting in cell viabilities and transfection efficiencies >90 percent, which exceed those of other transfection methods.

 

 

Transfection Efficiency and Cell Viability >90%  

 

Cells were transfected with pGFP DNA using the appropriate pre-loaded protocol on the MaxCyte STX. 24-48 hours post transfection cells were examined for cell viability and transfection efficiency. *SF9 results at 72 hours.

Efficient Delivery of Any Molecule

 

Flow Electroporation Technology is a universal technology able to deliver any molecule(s), regardless of size, to cells – DNA, mRNA, gRNA, siRNA, nucleic acid/protein complexes, proteins and cell lysates enabling endless applications.

 

Large Chromosomal DNA Delivery

 

Efficient Delivery of an Artificial Chromosome. Human foreskin fibroblasts were electroporated with artificial chromosome (100kb). Cells were selected with puromycin for two days starting at 48 hours post electroporation. Images taken at 28 days post electroporation.

Protein Delivery

Delivery of a Functional Protein Induces Direct Intra-Lineage Conversion. 3H mouse mesenchymal (10T1/2) cells were electroporated with MyoD and allowed to attach to culture dish overnight. Cells were cultured in 2% HS media for days and stained with a-MyHC Ab. High efficiency delivery of MyoD converted mouse MSC to differentiated muscle cells.

mRNA Delivery

Delivery of mRNA to Stem Cells. CD34+ hematopoietic stem cells were isolated and transfected with mRNA GFP to overcome hematopoietic cell toxicity issues that are faced when transfecting DNA plasmid.

Simple, Streamlined Process

 

The process for performing small- and large-scale electroporation are both completed in four easy steps: mixing of cells and loading agent, transferring to a processing assembly, electroporation and cell recovery.

Cell Preparation: Cells are harvested from culture using standard methods and suspended at high density (1-2×108 cells/mL) in MaxCyte Electroporation Buffer, a physiologically balanced salt solution that contains no biological agents. The same buffer is used for all cell types. Cells are mixed with loading agents (DNA, RNA, protein, etc.) and transferred to sterile, single use processing assemblies (PAs).
Electroporation: MaxCyte Scalable Transfection Systems come pre-loaded with a variety of electroporation protocols that are optimized for individual cell types. The user transfers the PA to the instrument, selects the appropriate protocol and PA type, and clicks the start icon to begin the run.
Post Electroporation Cell Handling: After electroporation, cells are transferred from the PA to a sterile, multi-well dish or T-flask and allowed to recover for 30-40 minutes at 37°C. The cells are suspended in standard cell medium and either cultured for immediate use or cryopreserved.

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