Insect Cells

Plasmid to Protein Within Days Using MaxCyte’s Delivery Platform

 

Insect cells offer an attractive means of biomanufacturing as they post-translationally modify proteins in a manner similar to that of mammalian cells, yet are easy to culture with simplified cell growth that is readily adapted to high-density suspension. While both transient transfection and recombinant baculovirus platforms are commonly used methods for insect cell protein expression, Flow Electroporation™ Technology offers a more rapid and straightforward means of insect-based protein production.

 

  • >90% cell viability and transfection efficiency for Sf9, Sf21 and SL3 cells
  • Requires no specialized constructs, viral stock production, engineered cells or media additives
  • Eliminates the need to use complex, time-consuming baculovirus expression systems
  • High-level protein production within three days
  • Simplifies downstream purification

 

 

High Efficiency Insect Cell Transfection for Rapid Protein Production

 

 

A variety of insect cells, including SF9 and SL3 cells, have been transfected using MaxCyte electroporation.

Cells were transfected with pGFP DNA using the appropriate pre-loaded protocol on the MaxCyte STX.  24-48 hours post transfection cells were examined for cell viability and transfection efficiency.

Eliminate Baculovirus for Streamlined Production

 

Baculovirus-mediated protein production remains an extended, multi-stage process. In contrast, Flow Electroporation™ Technology directly transfects Sf9, Sf21 and SL3 cells with >90% cell viability and transfection efficiency levels, allowing for rapid, high-level protein production within three days.

 

Simplified Workflow Using Flow Electroporation Technology. Direct, high-quality transfection of insect cells enables high-titer protein production within days.

VLP Production Within Days While Eliminating Baculovirus Contamination

 

Sf9 cells were transfected with a plasmid encoding three VLP antigens. In tandem, a baculovirus (BV) expression system was used to produce VLPs containing the identical three antigens. SDS-PAGE analysis of cell supernatants (48 hours post transfection/infection) shows the presence of the three VLP antigens in all electroporation and BV samples; however, BV protein contaminants were also present in supernatants from BV-infected cells, creating purification challenges and yield loss.

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Protein Production using the MaxCyte Platform

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