Primary & Stem Cells

Developed from the ground up for exceptional primary cell-engineering.

 

Over 15 years ago we developed a non-viral delivery platform for cell-engineering that fulfilled the stringent demands of clinical use, including the ability to reproducibly modify primary human cells with high efficiency and low cytotoxicity. Today, these attributes enable the engineering of primary and stem cells for a variety of clinical and research applications.

  • Hematopoietic cells (T cells, B-cells, Macrophages, Dendritic cells, NK cells)
  • Stem cells
  • Neurons
  • Myoblast
  • Epithelial cells
  • Skeletal muscle cells
  • Fibroblasts

 

 

High-Performance Transfection of a Variety of Human Primary Cells

 

 Primary cells were transfected and transfection efficiency and viability assessed 24 hours post electroporation.

Primary Neurons

 

Exogenous Protein Expression in Primary Neuronal Cells. E18 rat hippocampal, cortical and ventricular neurons were electroporated and plated at 5×105 cells/cm2 in multi-well plates. Five days post EP cells were assayed for cell viability and GFP expression.

Human Skeletal Muscle Cells

 

Highly Efficient Transfection of Primary Cells. Human Skeletal Muscle Cells (hSkMCs) were isolated from adult biopsy samples and transfected with pGFP. Cells were either examined one day post electroporation (fresh) or cryopreserved and examined one day following cell thawing

Mesenchymal Stem Cells (MSCs)

Mouse MSCs Converted to Differentiated Muscle Cells Through Delivery of MyoD. 3H mouse mesenchymal (10T1/2) cells were electroporated with MyoD and allowed to attach to culture dish overnight. Cells were cultured in 2% HS media for days and stained with a-MyHC Ab. High efficiency delivery of MyoD converted mouse MSC to differentiated muscle cells.

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