Choosing a host system for the expression of recombinant proteins should be carefully evaluated prior to the initiation of any biotherapetuic development programs. Since different hosts express proteins with different efficiencies and with different posttranslational modifications, changing hosts during the course of development may impact the expected activity of the protein and may require repeated studies or additional process development, delaying the time to market. Scientists must also consider the expression system’s productivity, consistency, and the current regulatory environment. This paper includes a commentary on maintaining the same host system throughout the development process, lays out a brief review of key references examining the differences in glycosylation in HEK versus CHO cells, and provides a more in-depth list of related literature.
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