Application Notes & Technical Papers

Protein Production in Insect Cells Using Flow Electroporation: A Superior Alternative to Baculovirus Expression

Insect cells are used for the industrial manufacturing of many products that are currently in clinical trials or already available on the market for veterinary and human applications. These cells offer an attractive means of protein production as they post-translationally modify proteins in a manner similar to that of mammalian cells. Furthermore, they are easy to culture with simplified cell growth that is readily adapted to high-density, suspension-based growth in serum- and protein-free media. Although a variety of transient transfection methods and recombinant baculovirus platforms are regularly used for insect cell protein expression (1, 2, 3), not all of these systems fulfill the scalability and reproducibility requirements for biomanufacturing.

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