Reduce assay development time to days by eliminating the need for stable cell lines. Easily overexpress GPCRs of interest or fusion proteins, artificially couple receptors to specific G alpha subunits or knock out signaling components in your cells of choice. Cryopreserve cells post transfection to create an assay ready cell bank without sacrificing performance.
Functional, cell-based GPCR assays
Second messenger assays including cAMP, IP3, & FLIPR®
High throughput screening
High content screening
MaxCyte Transfection systems use a single buffer for all cell types and come pre-loaded with cell-type specific electroporation protocols. All you need to do to develop your assay is perform a DNA titration experiment. By altering the concentration of the target expression plasmid used for transfection, researchers can easily control target expression levels and fine tune assay sensitivity.
High content and high throughput campaigns require a large number of cells to perform a single screen. Other transfection technologies require multiple small-scale transfections, re-optimization of transfection protocols, and/or bulk usage of costly transfection agents. MaxCyte Flow Electroporation™ technology easily scales from assay development to full library screening and creation of assay ready cell banks. Migration from small-scale to bulk transfection is seamless, requiring no further optimization.
By using MaxCyte-driven transient GPCR expression, researchers can reduce the time and/cost of securing stable cell lines, move to screen faster, and perform more reliable assays by avoiding the drift in GPCR expression levels of stable cell lines
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