Rapidly Create and Perform Quality Kinase Assays


Consistently overexpress kinases or kinase substrates for rapid kinase-targeted drug discovery and development. Easily scale from assay development to full high content or high throughput screening campaigns or create assay ready cell banks via bulk large volume transfections.

  • Eliminates the need for stable cell lines
  • >90% transfection efficiency and >90% cell viability for most cell types
  • Use physiologically relevant cell types including primary or stem cells
  • Computer-controlled for reduced day-to-day and user-to-user assay variability
  • Rapid assay development
  • Fully scalable, able to support assay development through large screening campaigns


High Content Kinase Screening

Cells were transfected with a plasmid encoding eGFP expressed as a fusion protein with tandom PI3P binding domains (2XFYVE).  Active PI3 kinase leads to localization of GFP to endosomes and visualized as granules.  PI3K inhibition leads to redistribution of fluorescence throughout the cytoplasm.  Transiently transfected cells or cells stably expressing eGPF-2XFYVE were incubated for 30 minutes with various concentrations of wortmannin.  PI3K activity was assessed using high content screening to visualize granule localization.


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