Our scientists have developed several cryopreservation protocols that enable cell archiving while maximizing cell viability and target expression upon thawing. Transfected cells can be cryopreserved following a short 20-minute incubation period or cultured for several hours to overnight prior to cryopreservation. Specific cell types may have slightly increased viability and transfection efficiency using specific cryopreservation conditions, however, overall cell health and transgene expression are not significantly affected. This provides an additional level of flexibility in cell handling post transfection and scheduling of downstream cell applications.
The quality of transfected cells post cryopreservation translates into the maintenance of gene expression and performance in downstream cell-based assays. Researchers can easily create a bank of assay-ready cells following a single large scale transfection providing an additional level of screen-to-screen reproducibility.