Insect cells offer an attractive means of biomanufacturing as they post-translationally modify proteins in a manner similar to that of mammalian cells, yet are easy to culture with simplified cell growth that is readily adapted to high-density suspension. While both transient transfection and recombinant baculovirus platforms are commonly used methods for insect cell protein expression, Flow Electroporation™ Technology offers a more rapid and straightforward means of insect-based protein production.
A variety of insect cells, including SF9 and SL3 cells, have been transfected using MaxCyte electroporation.
Baculovirus-mediated protein production remains an extended, multi-stage process. In contrast, Flow Electroporation™ Technology directly transfects Sf9, Sf21 and SL3 cells with >90% cell viability and transfection efficiency levels, allowing for rapid, high-level protein production within three days.