Flow Electroporation™ Technology scales in two ways – from small- to large-scale on a single instrument and from one MaxCyte Scalable Transfection System to another.
Other transfection technologies require multiple small-scale transfections, reoptimization of transfection protocols and/or bulk usage of costly reagents for larger scale transfection. MaxCyte electroporation seamlessly scales requiring no further assay optimization with transfection quality and cell performance in downstream applications unaffected by scale up.
Small-scale electroporation is conducted using an OC-100, OC-400 or CL-1.1 processing assembly (PA). The PA is loaded on a MaxCyte Scalable Transfection System and the entire aliquot of cells is electroporated within seconds. A CL-2 (MaxCyte STX) or VLXD (MaxCyte VLX) PA is used for large-scale electroporation in which 3 mL fractions flow in succession through the electroporation chamber and continue into the cell collection bag. Up to 2×1011 cells can be transfected in < 30 minutes.